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Plasma From Recovered COVID-19 Patients Inhibits Spike Protein Binding to ACE2 in a Microsphere-Based Inhibition Assay.
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- Author(s): Gniffke, Edward P (); Harrington, Whitney E (); Dambrauskas, Nicholas (); Jiang, Yonghou (); Trakhimets, Olesya (); Vigdorovich, Vladimir (); Frenkel, Lisa (); Sather, D Noah (); Smith, Stephen E P ()
- Source:
Journal of Infectious Diseases. 12/15/2020, Vol. 222 Issue 12, p1965-1973. 9p.
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- Abstract:
We present a microsphere-based flow cytometry assay that quantifies the ability of plasma to inhibit the binding of spike protein to angiotensin-converting enzyme 2. Plasma from 22 patients who had recovered from mild coronavirus disease 2019 (COVID-19) and expressed anti-spike protein trimer immunoglobulin G inhibited angiotensin-converting enzyme 2-spike protein binding to a greater degree than controls. The degree of inhibition was correlated with anti-spike protein immunoglobulin G levels, neutralizing titers in a pseudotyped lentiviral assay, and the presence of fever during illness. This inhibition assay may be broadly useful to quantify the functional antibody response of patients recovered from COVID-19 or vaccine recipients in a cell-free assay system. [ABSTRACT FROM AUTHOR]
